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. Author manuscript; available in PMC: 2016 Jan 26.
Published in final edited form as: N Engl J Med. 2013 Nov 28;369(22):2105–2114. doi: 10.1056/NEJMoa1304603

Figure 2. ARMC5 Alterations in Leukocyte and Tumor DNA.

Figure 2

Panel A shows the two ARMC5 alterations detected in the tumor DNA obtained from 18 patients. The analysis of leukocyte DNA (left column) shows the germline alteration (blue box). Each tumor under “Somatic Variants in Different Tumors” is shown as a box with a blue rectangle (showing the alteration in leukocyte DNA) and a yellow rectangle (showing the alteration that is detected only in the tumor DNA). Germline DNA from 4 patients was not available (bottom right). Panel B shows Western blot analysis of ARMC5 protein in adrenocortical human cell line H295R transfected with control small interfering RNA (siRNA) or ARMC5 siRNA (left), 3 normal adrenal glands (middle), and 17 samples obtained from patients with corticotropin-independent macronodular adrenal hyperplasia (right): 7 without an ARMC5 alteration and 10 with various types of ARMC5 alterations. LOH denotes loss of heterozygosity, LT left tumor, and RT right tumor.