Figure 3.

Protective efficacy conferred by vaccination at birth in C57/BL6 mice. Groups of three to nine newborn C57/BL6 mice were vaccinated with MTBVAC, BCG or PBS. At eight weeks post-birth, animals were challenged intranasally with H37Rv and four weeks later CFUs were determined in lungs (A) and spleen (B). Data of two independent experiments are shown in the figure. Data in the graphs are represented as mean ± SEM. One-way ANOVA test with Bonferroni post analysis was performed to calculate statistical significance. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. P value shown in Experiment 2 (SPLEEN) graph, comparing BCG and MTBVAC groups, was determined using a Mann–Whitney t-student test.