Table 4.

Effect of EBN on the production of IgE, IgA, and IgM

Group	IgE	IgA	IgM
Control	4.08±0.13	8.74±0.71	31.35±4.83
LPS	5.43±0.1*	20.33±1.0**	84.37±16.39*
EBN	5.28±1.1*	17.28±1.0**	79.24±16.03*

Notes: Cells were plated at 3×10⁶ cells/well. A total of 20 μg LPS (5 μg/mL) or EBNE (0.38 mg/mL) was dissolved in media and incubated with the cells for 72 hours at 37°C with 5% CO₂. Following incubation, the concentrations of IgE, IgA, IgM in the supernatant were measured using a CBA Mouse Immunoglobulin Isotyping Kit. The data are the mean ± SD (n=3/treatment).

^*Significant difference from control group (P<0.05).

^**Significant difference from control group (P<0.01).

Abbreviations: LPS, lipopolysaccharide; EBN, Edible bird’s nest; EBNE, EBN extract; SD, standard deviation; CBA, cytometric bead array.