Table 5.

Effect of EBN on the production of IgG3, IgG1, IgG2a, and IgG2b

Group	IgG3	IgG1	IgG2a	IgG2b
Control	4.47±0.14	5.37±0.71	4.83±0.025	4.63±0.56
LPS	6.36±0.72**	8.35±1.21*	9.04±2.73*	10.63±4.91*
EBN	5.85±0.63*	7.79±1.63	6.87±1.14	6.51±1.32

Notes: Cells were plated at 3×10⁶ cells/well. A total of 20 μg LPS (5 μg/mL) or EBNE (0.38 mg/mL) was dissolved in media and incubated with the cells for 72 hours at 37°C with 5% CO₂. Following incubation, the concentrations of IgG3, IgG1, IgG2a, and IgG2b in the supernatant were measured using a CBA Mouse Immunoglobulin Isotyping Kit. The data are the mean ± SD (n=3/treatment).

^*Significant difference from control group (P<0.05).

^**Significant difference from control group (P<0.01).

Abbreviations: LPS, lipopolysaccharide; EBN, Edible bird’s nest; SD, standard deviation; CBA, cytometric bead array.