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. 2016 Jan 15;57(1):94–104. doi: 10.1167/iovs.15-18235

Figure 3.

Figure 3

Melatonin receptor 1 and MT2 deletion effects of P-AKT/AKT levels. Western blotting analysis of P-AKT and AKT (P and UnP) levels in retinas at different Zeitgeber Time (ZT) in 3 months C3H/f+/+ (A, D, G, J), C3H/f+/+MT1−/− (B, E, H, K), and C3H/f+/+MT2−/− mice (C, F, I, L). P-AKT (Thr308 and Ser473) levels were significantly higher in the late night (ZT19-22; P < 0.05, 1-way ANOVA, followed by Tukey test) in C3H/f+/+ but not in C3H/f+/+MT1−/− and C3H/f+/+MT2−/− mice. Densitometric quantification of P-AKT (Thr308 and Ser473) and AKT levels were performed on three independent retinal samples for each ZT. AKT: serine/threonine protein kinase.