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. 2016 Jan 19;9:377–386. doi: 10.2147/OTT.S96309

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© 2016 Wang et al. This work is published and licensed by Dove Medical Press Limited

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Cocultures and MTT for cell proliferation.

Notes: (A) The protein markers of SKOV3, A2780, and HOF. (B) EGFR was overexpressed in SKOV3 and A2780. (C) A2780 cells (red) were cocultured with HOF cells (green, copGFP or EGFR-copGFP), 10×. (D) SKOV3 cells (red) were cocultured with HOF cells (green, copGFP or EGFR-copGFP), 10×. (E) MTT assay of A2780 cells at 0, 24, 48, 72, and 96 hours after culture in medium. (F) MTT assay of SKOV3 cells at 0, 24, 48, 72, and 96 hours after culture in medium. *P<0.05.

Abbreviations: EGFR, epidermal growth factor receptor; EV, empty vector; GFP, green fluorescence protein; HOF, human ovarian fibroblasts; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide.