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. 2015 Nov 18;20(2):266–286. doi: 10.1111/jcmm.12718

Figure 3.

Figure 3

(A) Western blot analysis demonstrates the phosphorylation of ASK1, JNK, p38 and ERK1/2 without the alteration of their basal expression in response to the treatment of melanoma cells with imiquimod for 48 hrs. Actin was used as an internal control for loading and transfer. (B) Analyses of band intensity on films are presented as the relative ratio of phospho‐PERK, phosphor‐ASK1, phosph‐JNK, phosphor‐p38 and phosphor‐ERK1/2 to actin. Bars represent mean ± SD from three blots. *P < 0.05 versus control. EMSA demonstrates the induction of the DNA‐binding activities of the transcription factors AP‐1 (C and D), ATF‐2 (E and F), p53 (G and H) and ELK‐1 (I and J) in BLM and MV3 cells before and after the exposure to imiquimod for 48 hrs. Data are representative of three independent experiments.