Fig. 1.

Composite diagram showing binding of monoclonal antibody (mAb) Fab/Fab’ fragments to Bla g 2 (top) and Der p 1 (bottom). Top, binding of mAb 7C11 (PDB ID 2NR6; cyan) and mAb 4C3 (PDB ID 3LIZ; green). Carbohydrate residues bound to Asn268 of free Bla g 2 are yellow and repositioned in the complex with mAb 4C3 are blue. These sugars are involved in the antibody interactions with Bla g 2 (orange), extending the mAb 4C3 epitope. A carbohydrate moiety bound to Asn317 is located far from the epitope. Bottom, binding of Der p 1 (gray) with Fab fragments of mAb 4C1 (PDB ID 3RVW; blue), 10B9 (PDB ID 4PP2; red), and 5H8 (PDB ID 4PP1; orange). Epitopes recognized by the mAb 4C1 (cross-reactive with Der f 1), and the Der p 1-specific mAb 10B9 partially overlap. Catalytic Cys34 is shown in pink. Figure was prepared with Pymol (www.pymol.org)