Fig. 2.
HPLC-based activity profiling of the three selected plant extracts in the active time window (0–20 min). SunFire C18 column (150 × 10 mm i.d., 5 μm); 5–100% MeCN/0.1% aqueous formic acid for 30 min and 100% MeCN/0.1% aqueous formic acid for 5 min, 4 mL/min; time-based fractionation; detection: 200–500 nm, maxplot. A Casearia commersoniana (stems) MeOH extract. B Mosquitoxylum jamaicense (leaves) MeOH extract. C Combretum cacoucia (leaves) MeOH extract. The assay results are expressed as the radical scavenging capacity of the microfractions in the DPPH assay, compared to gallic acid as the positive control, and as cell death in the UV-B protection assay, as compared to the UV-B irradiated cells without the addition of fractions.