Fig. 2.

Effect of additives on the kinetics of α-synuclein aggregation. (A – C): Kinetic curves for fibril formation from α-synuclein (0.25 mg/mL, pH 7.5, 50 μg/mL heparin, 40 °C) in the presence of dopamine, dopamine-containing polymer (DP) and PEG 12. A – DP; B – dopamine; C PEG 12. Black – no additive, red–0.025% additive, green–0.2% additive, yellow–2% additive. (D–E): Initiation and elongation rates for fibril formation from α-synuclein in the presence of dopamine, DP and PEG 12. D – initiation rate (1/lag phase); E−fibril yield (ThT fluorescence). Black – dopamine, red – DP, green – PEG 12. Scale bars correspond to standard error between independent measurements. (F – I): EM images of aggregates obtained after 5 days of incubation. F – no additive; G – 0.5% DP; H – 0.5% dopamine; I – 0.5% PEG 12. Scale bars: 500 nm.(For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)