Fig. 4.
Inhibition of RhoA/ROCK-mediated actin polymerisation underlies cAMP-mediated repression of YAP/TAZ and TEAD. VSMCs were treated with RhoA inhibitor (C3 Transferase; 2 μg/ml), ROCK inhibitor (Y-27,632; 10 μM) or actin-polymerisation inhibitor (Latrunculin B; 5 μg/ml) for 30 min and phosphorylation of YAP/TAZ was quantified Western blotting (A–C). VSMCs were transfected with TEAD-luciferase plasmid (8xTEAD-LUC) or TnT-minimal promoter-luciferase plasmid (TnT-minP) and treated with indicated RhoA/ROCK pathway inhibitors for 8 h (D). Cells infected with either active RhoA (RhoAG14V) or control adenovirus were stimulated with 25 μM forskolin for 8 h (E). All data is n = 3. * indicates p < 0.05, ** indicates p < 0.01. ## indicates p < 0.01 and ### indicates p < 0.001 on log transformed data.