Fig. 6.

YAP and TAZ cooperate to regulate VSMC proliferation. VSMC were transfected with siRNA targeting YAP, TAZ or YAP plus TAZ. Total cell lysates were prepared 24 h post transfection and analysed by Western blotting for YAP, TAZ, phospho-Rb, GAPDH and ACTIN (A). Phospho-Rb levels were quantified by densitometry (B; n = 3). siRNA-transfected cells were labelled with BrdU between 24 and 36 h post transfection (C; n = 3). * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001.