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. 2016 Feb;147(2):175–188. doi: 10.1085/jgp.201511494

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© 2016 Kravtsova et al.

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Figure 7. — HS does not alter the functional interaction between nAChR and α2 Na,K-ATPase. (A–C) Changes in membrane potential induced by 100 nM nicotine in rat soleus muscle fibers in control (A), after 6 h of HS (B), and in the presence of ouabain (C). In A and B, junctional membrane (open circles), extrajunctional membrane (closed circles); horizontal bars indicate the periods when nicotine was present in the perfusate. In C, muscles were preincubated with the indicated concentration of ouabain for 30 min before nicotine addition. RMPs were recorded from extrajunctional membrane regions. Changes in RMP were calculated as the difference between the potential measured before and after 60 min of nicotine action. **, P < 0.01 compared with corresponding value in the absence (0 nM) of ouabain. Error bars indicate SEM.