Table 1.

Corrected Kinetic Constants for Reactions of KIAA1363 and CES1 with CPO or PO

		Kinetic parameters a
Enzyme	Oxon	kinact (s−1)	Ki c (M)	kinact/Ki (s−1M−1)	kreact (s−1)
KIAA1363	CPO	0.0069 ± 0.0007	1.6 × 10−9	4.4(±0.4) × 106	0.00043 ± 0.00019
CES1	CPO	— b	— b	6.7(±4.2) × 106	— b
KIAA1363	PO	0.00092	2.9 × 10−9	0.4 × 106	0.0003
CES1	PO	— b	— b	1.5 × 106	— b

^aValues are means ± SD of 2–4 independent experiments, each with triplicate reactions for each oxon concentration.

^bNot determined because curves were linear, not hyperbolic (see Fig. 6B for CPO data).

^cCorrected K_i = K_i′/(1 + [S]/K_m). [S] = 250 μM; K_m^4-MUBA = 227 μM