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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Acta Biomater. 2015 Nov 24;31:156–166. doi: 10.1016/j.actbio.2015.11.051

Figure 5. Cell Morphology Assessment.

Figure 5

Effects of SF hydrogel stiffness and TGF-β1 on attachment and spreading of hMSCs. (a–h) Confocal stack renderings of hMSCs with (e–h) or without (a–d) TGF-β1 at various SF hydrogel elasticities (SF1.5-6kPa, SF2-20kPa, SF3-33kPa, SF4-64kPa). Cells were stained for cell nuclei (DAPI, blue) and F-actin (phalloidin, green), scale bar 300 µm. (i) Initial hMSC morphologies after 24 h seeding imaged and presented in grayscale with respect to SF hydrogel stiffness and TGF-β1 administration; scale bar 50 µm. Cell density (j) and attachment (k) of hMSCs for all the hydrogel matrices shown in images a–h were quantified and tabulated. * P < 0.05, *** P < 0.001, NS P > 0.05.