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Assay distinguished wild-type KRAS gene codons 12 and 13 from different mutants using AS primers. Lane M: Low molecular weight DNA ladder; Lane 1: negative control; Lane 2: wild-type; Lane 3: G12S mutant; Lane 4: G12R mutant; Lane 5: G12C mutant; Lane 6: G12D mutant; Lane 7: G12A mutant; Lane 8: G12V mutant; Lane 9: G13D mutant.
