Table 1. Primers used in MAS-PCR for detecting the most common mutations in codons 12 and 13 of KRAS gene.

Primer	Sequence	Conc. (μM)	Product length (bp)
KRAS-F	5’-GGCCTGCTGAAAATGACTGAA-3’	0.05	113 bp
KRAS-R	5’-GGATCATATTCGTCCACAAAATG-3’	0.075	113 bp
G12S-R	5’-CACTCTTGCCTACGCCACT-3’	0.05	64 bp
G12R-F	5’-TTGTGGTAGTTGGAGCTC-3’	0.025	85 bp
G12C-F	5’-CTGAATATAAACTTGTGGTAGTTGGAGCTT-3’	0.01	97 bp
G12D-F	5’- ATAAACTTGTGGTAGTTGGAGCTGA-3’	0.02	91 bp
G12A-F	5’- GTGGTAGTTGGAGCTGC-3’	0.02	83 bp
G12V-R	5’- AAGGCACTCTTGCCTACGCCAA-3’	0.02	68 bp
G13D-F	5’- AAACTTGTGGTAGTTGGAGCTGGTGA-3’	0.0125	89 bp