Table 2. KRAS mutational status in 270 CRC patients.
| KRAS status | Type of mutations Amino acid change | Pyrosequencing Number of mutations (%) | MAS-PCR Number of mutations (%) |
|---|---|---|---|
| Mutant | All Codon 12 and 13 | 120/270 | 113/270 |
| (44.44%) | (41.85%) | ||
| Mutated Codon 12 | All Codon 12 | 61/120 | 54/113 |
| (50.83%) | (47.79%) | ||
| G12D | GGT>GAT | 31/120 | 28/113 |
| Gly→Arg | (25.83%) | (24.78%) | |
| G12V | GGT>GTA | 15/120 | 15/113 |
| Gly→Val | (12.50%) | (13.27%) | |
| G12A | GGT>GCT | 7/120 | 4/113 |
| Gly→Ala | (5.83%) | (3.54%) | |
| G12S | GGT>AGT | 3/120 | 3/113 |
| Gly→Ser | (2.50%) | (2.65%) | |
| G12C | GGT>TGT | 4/120 | 4/113 |
| Gly→Cys | (3.33%) | (3.54%) | |
| G12R | GGT>CGT | 1/120 | 0/113 |
| Gly→Arg | (0.83%) | ND | |
| G13D | GGC>GAC | 59/120 | 59/113 |
| Gly→Asp | (49.17%) | (52.21%) |
Underlined bases represent the substitutions in the respective codon.
ND denotes not detected.