Figure 1.

Lipin Dysfunction Leads to NE and ER Expansion Both in S. pombe and S. japonicus

(A) A schematic diagram representing lipid biosynthesis pathways downstream of phosphatidic acid. CDP-DAG, cytidine diphosphate diacylglycerol; DAG, diacylglycerol; PA, phosphatidic acid; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PI, phosphatidylinositol; PS, phosphatidylserine; TAG, triacylglycerol.

(B and C) Fluorescence confocal images of S. pombe (B) and S. japonicus (C) cells of the indicated genotypes co-expressing the ER luminal marker GFP-ADEL and the high mobility group protein Nhp6-mCherry marking nucleoplasm. The Nhp6-mCherry images are inverted. Nuclear circularity indices are presented as Θ values (for both S. pombe and S. japonicus: WT, n = 51 cells; ned1Δ, n = 50 cells; spo7Δ, n = 50 cells; and nem1Δ, n = 50 cells).

The scale bars represent 5 μm. See also Figure S1.