Fig 5. Effect of cycloheximide on development of tko25t and wild-type flies.
(A) Means ± SD of times to eclosion of flies of the sex and genotypes indicated, on high-sugar medium, with or without cycloheximide at the indicated concentrations. Based on pairwise t tests, and considering all the flies of a given sex and genotype cultured at a specific drug concentration as a single population, mean eclosion times were significantly different (p < 0.05) at different cycloheximide concentrations, apart from tko25t flies of either sex at 100 μg/ml, which were not different from tko25t flies cultured in the complete absence of the drug. (B) Pooled eclosion data from four independent experiments conducted with different concentration ranges of cycloheximide. Female developmental delay showed consistent decrease with increasing cycloheximide concentration. Males showed same trend (Panel C in S5 Fig).