Figure 5. Elimination of PhLP1 in striatal neurons significantly impairs expression and function of AC5-Golf complex.
(A) Representative immunoblot data of AC5, different G protein subunits and RGS proteins in stratal tissues from adult control or Pdcl cKO mice. (B) Quantification of protein levels. Data were normalized to the percentage of protein level in control mice. **p<0.01, ***p<0.001, Student’s t-test, n = 3 mice. (C) mRNA quantification in striatum tissue from control and Pdcl cKO mice. Data were normalized to the percentage of mRNA level in control mice. (D), Basal cAMP level was reduced in the striatum tissue of Pdcl cKO mice. ***p<0.001, Students’ t-test, n = 3 mice. (E) Striatal membrane adenylyl cyclase activity is reduced in Pdcl cKO mice. Adenylyl cyclase activity was measured either under basal conditions (without stimulation), or in the presence of forskolin (FSK, 1 μM), D1R specific agonist SKF38391 (SKF, 10 μM) or A2AR agonist CGS21680 (CGS, 10 μM). *p<0.05, **p<0.01, Student’s t-test, n = 3 reactions.