Fig. 2.

MICA expression on the plasma membrane and release of sMICA in human melanoma cell lines with different MICA-129 genotypes. a The pmMICA expression of human melanoma cell lines carrying the different MICA-129 genotypes (Met/Met, Met/Val, and Val/Val) was analyzed by flow cytometry in control cells (−SAHA) and cells treated with the HDAC inhibitor SAHA (10 μM) for 20 h before analysis (+SAHA). The MFI of pmMICA is shown as means plus SD (n ≥ 3). b In parallel, the amounts of soluble MICA (sMICA) in the supernatants were determined by ELISA and are shown as means plus SD of sMICA (pg/10⁶ cells). d, e The data were grouped according to the MICA-129 genotype, and cell lines carrying one or two MICA-129Met alleles (Met/X, i.e., Met/Met and Met/Val) were compared to cell lines which were homozygous for the MICA-129Val allele. The data are displayed as means plus SEM, and they were analyzed by t tests