Fig. 6.

Correlation of MICA mRNA and pmMICA expression intensity in L cells expressing the MICA-129Met and MICA-129Val variants. a The MFI of pmMICA expression was determined by flow cytometry on L-MICA-129Met (n = 14) and L-MICA-129Val (n = 12) clones. The data are summarized as means plus SD and were compared by a t test. b In parallel, the MICA mRNA expression (ΔCt_tg−hkg) was determined by qPCR. Expression values of the target gene (tg) MICA were calculated from mean of technical triplicates after normalization to the housekeeping gene (hkg) Hprt. The data are summarized as means plus SD and were compared by a t test. It should be noted that lower ΔCt_tg−hkg values indicate higher mRNA expression levels. The linear regressions of MICA mRNA expression and pmMICA expression are displayed for c L-MICA-129Met and d L-MICA-129Val clones. The coefficient of determination (R ²), the regression coefficient (reg. coeff.), and the P value (for the Pearson correlation) are indicated for both MICA variants