Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1993 Sep 1;90(17):8033–8037. doi: 10.1073/pnas.90.17.8033

Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells.

J C Burns 1, T Friedmann 1, W Driever 1, M Burrascano 1, J K Yee 1
PMCID: PMC47282  PMID: 8396259

Abstract

The restricted host-cell range and low titer of retroviral vectors limit their use for stable gene transfer in eukaryotic cells. To overcome these limitations, we have produced murine leukemia virus-derived vectors in which the retroviral envelope glycoprotein has been completely replaced by the G glycoprotein of vesicular stomatitis virus. Such vectors can be concentrated by ultracentrifugation to titers > 10(9) colony-forming units/ml and can infect cells, such as hamster and fish cell lines, that are ordinarily resistant to infection with vectors containing the retroviral envelope protein. The ability to concentrate vesicular stomatitis virus G glycoprotein pseudotyped vectors will facilitate gene therapy model studies and other gene transfer experiments that require direct delivery of vectors in vivo. The availability of these pseudotyped vectors will also facilitate genetic studies in nonmammalian species, including the important zebrafish developmental system, through the efficient introduction and expression of foreign genes.

Full text

PDF
8035

Images in this article

8034Fig. 1
on p.8034

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Conti C., Mastromarino P., Riccioli A., Orsi N. Electrostatic interactions in the early events of VSV infection. Res Virol. 1991 Jan-Feb;142(1):17–24. doi: 10.1016/0923-2516(91)90023-v. [DOI] [PubMed] [Google Scholar]
  2. Cornetta K., Anderson W. F. Protamine sulfate as an effective alternative to polybrene in retroviral-mediated gene-transfer: implications for human gene therapy. J Virol Methods. 1989 Feb;23(2):187–194. doi: 10.1016/0166-0934(89)90132-8. [DOI] [PubMed] [Google Scholar]
  3. Culp P., Nüsslein-Volhard C., Hopkins N. High-frequency germ-line transmission of plasmid DNA sequences injected into fertilized zebrafish eggs. Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):7953–7957. doi: 10.1073/pnas.88.18.7953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Emi N., Friedmann T., Yee J. K. Pseudotype formation of murine leukemia virus with the G protein of vesicular stomatitis virus. J Virol. 1991 Mar;65(3):1202–1207. doi: 10.1128/jvi.65.3.1202-1207.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Florkiewicz R. Z., Smith A., Bergmann J. E., Rose J. K. Isolation of stable mouse cell lines that express cell surface and secreted forms of the vesicular stomatitis virus glycoprotein. J Cell Biol. 1983 Nov;97(5 Pt 1):1381–1388. doi: 10.1083/jcb.97.5.1381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goff S., Traktman P., Baltimore D. Isolation and properties of Moloney murine leukemia virus mutants: use of a rapid assay for release of virion reverse transcriptase. J Virol. 1981 Apr;38(1):239–248. doi: 10.1128/jvi.38.1.239-248.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Graham F. L., Smiley J., Russell W. C., Nairn R. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J Gen Virol. 1977 Jul;36(1):59–74. doi: 10.1099/0022-1317-36-1-59. [DOI] [PubMed] [Google Scholar]
  8. Houdebine L. M., Chourrout D. Transgenesis in fish. Experientia. 1991 Sep 15;47(9):891–897. doi: 10.1007/BF01929879. [DOI] [PubMed] [Google Scholar]
  9. Lefrancios L., Lyles D. S. The interactionof antiody with the major surface glycoprotein of vesicular stomatitis virus. I. Analysis of neutralizing epitopes with monoclonal antibodies. Virology. 1982 Aug;121(1):157–167. [PubMed] [Google Scholar]
  10. Levinson W., Oppermann H., Rubenstein P., Jackson J. Host range restriction of vesicular stomatitis virus on duck embryo cells. Virology. 1978 Apr;85(2):612–616. doi: 10.1016/0042-6822(78)90466-x. [DOI] [PubMed] [Google Scholar]
  11. Marsh M., Helenius A. Virus entry into animal cells. Adv Virus Res. 1989;36:107–151. doi: 10.1016/S0065-3527(08)60583-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mastromarino P., Conti C., Goldoni P., Hauttecoeur B., Orsi N. Characterization of membrane components of the erythrocyte involved in vesicular stomatitis virus attachment and fusion at acidic pH. J Gen Virol. 1987 Sep;68(Pt 9):2359–2369. doi: 10.1099/0022-1317-68-9-2359. [DOI] [PubMed] [Google Scholar]
  13. Miller A. D., Buttimore C. Redesign of retrovirus packaging cell lines to avoid recombination leading to helper virus production. Mol Cell Biol. 1986 Aug;6(8):2895–2902. doi: 10.1128/mcb.6.8.2895. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Miller A. D., Garcia J. V., von Suhr N., Lynch C. M., Wilson C., Eiden M. V. Construction and properties of retrovirus packaging cells based on gibbon ape leukemia virus. J Virol. 1991 May;65(5):2220–2224. doi: 10.1128/jvi.65.5.2220-2224.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Miller A. D., Law M. F., Verma I. M. Generation of helper-free amphotropic retroviruses that transduce a dominant-acting, methotrexate-resistant dihydrofolate reductase gene. Mol Cell Biol. 1985 Mar;5(3):431–437. doi: 10.1128/mcb.5.3.431. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Miyanohara A., Elam R. L., Witztum J. L., Friedmann T. Long-term transgene expression from genetically modified hepatocytes grafted to the rat liver. New Biol. 1992 Mar;4(3):261–267. [PubMed] [Google Scholar]
  17. Powers D. A., Hereford L., Cole T., Chen T. T., Lin C. M., Kight K., Creech K., Dunham R. Electroporation: a method for transferring genes into the gametes of zebrafish (Brachydanio rerio), channel catfish (Ictalurus punctatus), and common carp (Cyprinus carpio). Mol Mar Biol Biotechnol. 1992 Aug-Oct;1(4-5):301–308. [PubMed] [Google Scholar]
  18. Roman L. M., Scharm A., Howell K. E. Immunoselection of stably transformed MDCK cells expressing the vesicular stomatitis virus G-protein at the basolateral surface. Exp Cell Res. 1988 Apr;175(2):376–387. doi: 10.1016/0014-4827(88)90201-7. [DOI] [PubMed] [Google Scholar]
  19. Schloemer R. H., Wagner R. R. Mosquito cells infected with vesicular stomatitis virus yield unsialylated virions of low infectivity. J Virol. 1975 Apr;15(4):1029–1032. doi: 10.1128/jvi.15.4.1029-1032.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Simonsen C. C., Levinson A. D. Isolation and expression of an altered mouse dihydrofolate reductase cDNA. Proc Natl Acad Sci U S A. 1983 May;80(9):2495–2499. doi: 10.1073/pnas.80.9.2495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Streisinger G., Walker C., Dower N., Knauber D., Singer F. Production of clones of homozygous diploid zebra fish (Brachydanio rerio). Nature. 1981 May 28;291(5813):293–296. doi: 10.1038/291293a0. [DOI] [PubMed] [Google Scholar]
  22. Stuart G. W., McMurray J. V., Westerfield M. Replication, integration and stable germ-line transmission of foreign sequences injected into early zebrafish embryos. Development. 1988 Jun;103(2):403–412. doi: 10.1242/dev.103.2.403. [DOI] [PubMed] [Google Scholar]
  23. Toyoshima K., Vogt P. K. Enhancement and inhibition of avian sarcoma viruses by polycations and polyanions. Virology. 1969 Jul;38(3):414–426. doi: 10.1016/0042-6822(69)90154-8. [DOI] [PubMed] [Google Scholar]
  24. Weiss R. A., Boettiger D., Murphy H. M. Pseudotypes of avian sarcoma viruses with the envelope properties of vesicular stomatitis virus. Virology. 1977 Feb;76(2):808–825. doi: 10.1016/0042-6822(77)90261-6. [DOI] [PubMed] [Google Scholar]
  25. Wilson C., Reitz M. S., Okayama H., Eiden M. V. Formation of infectious hybrid virions with gibbon ape leukemia virus and human T-cell leukemia virus retroviral envelope glycoproteins and the gag and pol proteins of Moloney murine leukemia virus. J Virol. 1989 May;63(5):2374–2378. doi: 10.1128/jvi.63.5.2374-2378.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Winkler C., Vielkind J. R., Schartl M. Transient expression of foreign DNA during embryonic and larval development of the medaka fish (Oryzias latipes). Mol Gen Genet. 1991 Apr;226(1-2):129–140. doi: 10.1007/BF00273596. [DOI] [PubMed] [Google Scholar]
  27. Závada J. Pseudotypes of vesicular stomatitis virus with the coat of murine leukaemia and of avian myeloblastosis viruses. J Gen Virol. 1972 Jun;15(3):183–191. doi: 10.1099/0022-1317-15-3-183. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES