Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Dec 1;8(12):1569–1578. doi: 10.1242/dmm.021428

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 1. — Characterization of the DM1 heart-dysfunction phenotype in flies. Representative fluorescent confocal images of adult heart cells from flies expressing short [CUG(20)×] or long [CUG(250)×] repeats under the control of GMH5-Gal4. (A-C) In cardiomyocytes expressing CUG(20)×, revealed by anti-GFP antibody (red, B), Muscleblind signal (green, A) was dispersed in the nuclei and cytoplasm. (D-I) Combined immunodetection of Muscleblind (green, D and G) and FISH to detect ribonuclear foci (red, E and H) revealed dispersed expression of Muscleblind and absence of foci in flies expressing short CUG repeats (D-F). However, in flies expressing long CUG repeats (G-I), Muscleblind colocalized with ribonuclear foci (arrows). (J,K) Representative confocal stacks of phalloidin (red)-stained spiral fibers in the region surrounding the ostia in adult hearts (posterior A2-anterior A3 segment) reveals details of the heart structure, in particular increased fiber disorganization in GMH5-Gal4›CUG(250)× flies. Arrowhead points to ostia-associated nuclei. Merged images in C,F,I,J and K include DAPI (blue) counterstaining of the nuclei. All images are from 7-day-old flies. Scale bars: 10 µm.