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. 2015 Dec 1;8(12):1517–1529. doi: 10.1242/dmm.021972

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — Conditional deletion of Tsc1 during eye development leads to an activation of the mTORC1 pathway. (A) Immunoblot analysis of hamartin, total S6, pS6 (S235/236), pS6 (S240/244) and GAPDH from control, Lhx2-Cre:Tsc1^+/f and Lhx2-Cre:Tsc1^f/f eye extracts. (B) Densitometry analysis demonstrates that conditional deletion of Tsc1 results in a reduction of hamartin level and a parallel upregulation in pS6 (S235/236) and pS6 (240/244) levels. GAPDH was used for normalization of hamartin densitometry data. Total S6 was used for normalization of pS6 (S235/235) and pS6 (S240/244) densitometry data. All data represent the mean±s.e.m. of three mice from each genotype. Abbreviations: GAPDH, glyceraldehyde 3-phosphate dehydrogenase; kDa, kilodalton. P-values are denoted as follows: ns, not significant, *P≤0.05, ***P≤0.001, ****P≤0.0001.