Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Dec 1;8(12):1603–1614. doi: 10.1242/dmm.021998

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 4. — Apical-basal polarity is required for engulfment. (A-U) Egg chambers from starved flies stained with DAPI (cyan), anti-aPKC (yellow) and anti-Dlg (red). (A-C) Healthy, phase 3 and phase 5 control (tub-Gal80/+; GR1-GAL4/UAS-eGFP^dsRNA) egg chambers show follicle cell (FC) enlargement and maintain apical localization of aPKC as engulfment progresses. (D-F) aPKC^dsRNA healthy, phase 3 and phase 5 egg chambers show a reduced amount of aPKC localization, minimal FC enlargement, and defective engulfment. (G-I) baz^dsRNA healthy and phase 5 egg chambers show a reduced amount of aPKC localization, and defective engulfment, whereas phase 3 egg chambers show increased, but mislocalized, aPKC. (J-L) par-6^dsRNA healthy, phase 3 and phase 5 egg chambers show a loss in aPKC localization, no FC enlargement, and defective engulfment. Premature migration of FCs to the posterior of the egg chamber was seen in some healthy par-6 egg chambers (J; arrowhead). Arrows in G and J indicate gaps in the follicle cell layer. (M-O) crb^dsRNA healthy, phase 3 and phase 5 egg chambers show normal aPKC localization initially but, as engulfment progresses, aPKC localization is lost and the egg chambers are engulfment-defective. Arrows in E, H, K, N show regions without FC enlargement. (P-R) Egg chambers expressing Dhc^dsRNA show aPKC localization in phase 3 egg chambers; however, the localization is lost by phase 5 and egg chambers are engulfment-defective. (S-U) Egg chambers expressing Khc^dsRNA show normal aPKC localization and do not have engulfment defects. (V) Quantification of unengulfed germline for control, aPKC^dsRNA and crb^dsRNA egg chambers. (W) Quantification of the number of Dcp-1-positive particles in phases 1-4 for control, aPKC^dsRNA and crb^dsRNA egg chambers. At least three egg chambers were quantified for each genotype, phase and quantification method in V,W. 51 egg chambers were quantified for the control, 35 for aPKC^dsRNA and 30 for crb^dsRNA. All data are mean±s.e.m. One-way ANOVA and Bonferroni-Holm post-hoc tests were performed: ***P<0.005, *P<0.05, n.s., non-significant. Scale bar: 50 µm.