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. 2015 Dec 1;8(12):1603–1614. doi: 10.1242/dmm.021998

Fig. 5.

Fig. 5.

Cell-polarity knockdown egg chambers are defective for integrin enrichment and engulfment. (A-R) Egg chambers from starved flies stained with DAPI (cyan), anti-αPS3 (green) and anti-βPS (magenta). 4× zooms show the αPS3 channel of the boxed regions in the phase 3 images. Arrows in 4× zooms indicate the apical surface of the follicle cells. (A-C) Healthy, phase 3 and phase 5 control (tub-Gal80/+; GR1-GAL4/UAS-eGFPdsRNA) egg chambers show apical enrichment of αPS3/βPS as engulfment progresses. (D-O) Egg chambers expressing dsRNA against the apical determinants all show disrupted localization of αPS3. Arrowhead in J points to a double layer of FCs in a par-6 healthy egg chamber. (P-R) Egg chambers expressing Dhc64CdsRNA show initial apical αPS3 enrichment (Q,Q′), which is later lost (R). (S-U) Quantification of αPS3 in the apical and basal regions, and within the cytoplasm, for control, aPKCdsRNA and crbdsRNA egg chambers. The high temperature (29°C) might inhibit normal αPS3/βPS enrichment somewhat compared to 25°C (Fig. 1). All data are mean±s.e.m. At least three egg chambers were quantified for each genotype and phase in S-U. 62 egg chambers were quantified for the control, 38 for aPKCdsRNA and 38 for crbdsRNA. One-way ANOVA and Bonferroni-Holm post-hoc tests were performed: ***P<0.005, **P<0.01, n.s., non-significant. Scale bar: 50 µm.