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. 2015 Dec 1;8(12):1635–1641. doi: 10.1242/dmm.022020

Fig. 3.

Fig. 3.

Cell lines derived from mice fed a CDE diet express LPC markers. The LPC line, BMOL3, was derived from a mouse fed a 67% CDE diet. Immunofluorescence was used to detect known LPC markers [EpCAM (A,E), E-cadherin (B,F) and CK19 (C,G)] and mesenchymal marker vimentin (D,H). Representative images are shown without (A-D) and with (E-H) DAPI staining. Control staining with either Alexa-Fluor-488 goat-anti-rabbit or -594 goat-anti-rat alone are shown (I,J). Scale bars: 100 µm.