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. 2015 Oct 9;4(11):1400–1409. doi: 10.1242/bio.012617

Fig. 4.

Fig. 4.

Tnfrsf11b was regulated by core clock genes in MC3T3-E1 cells. (A) Bmal1-knockdown by siRNA in MC3T3-E1 cells. MC3T3-E1 cells were treated with Bmal1 siRNA (siRNA-Bmal1) or non-silencing RNA (siRNA-Negative), followed by further cultivation for 30 h (left panel) and 48 h (right panel) and the subsequent determination of Tnfrsf11b mRNA levels by real time qRT-PCR. Each value represents the mean±s.e.m. of three separate experiments. *P<0.05, significantly different from each control value. A representative result of three individual experiments is shown. (B) Effects of the overexpression of Bmal1 and CLOCK in MC3T3-E1 cells. MC3T3-E1 cells were transiently transfected with the expression vectors of Bmal1 and Clock, followed by further cultivation for 48 h and the subsequent determination of Tnfrsf11b levels by real time qRT-PCR. Relative mRNA expression was normalized to Gapdh expression. Each value represents the mean±s.e.m. of three separate experiments. *P<0.05, significantly different from each control value. (C) Per2-knockdown by siRNA in MC3T3-E1 cells. MC3T3-E1 cells were treated with Per2 siRNA (siRNA-Per2) or non-silencing RNA (siRNA-Negative), followed by further cultivation for 48 h and the subsequent determination of Tnfrsf11b mRNA levels by real time qRT-PCR. Each value represents the mean±s.e.m. of three separate experiments. *P<0.05, significantly different from each control value. A representative result of three individual experiments is shown.