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. 2015 Oct 12;4(11):1462–1472. doi: 10.1242/bio.013235

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Acellular porcine matrices seeded with pluripotent stem cells. (A) H9 human pluripotent stem cells (hPSCs) are able to reseed acellular porcine matrix but do not penetrate into the matrix very well as examined after 4 weeks post seeding. (B) Some stem cells that were seeded onto the matrix expressed Ecad when analyzed 4 weeks post seeding suggesting that at least a portion of the hPSCs had begun to differentiate. (C) hPSC-seeded porcine matrices were analyzed by qRT-PCR at different time point to examine the expression of stem cell, endoderm, mesoderm, and ectoderm markers. From this analysis, it does not appear that porcine matrix drives hPSCs in any specific direction of differentiation. (D) Cells were examined at different stages during the development of HIOs to provide a reference for part C. As expected, pluripotency markers are low and endodermal markers go up during the development process. Note scale differences of the y-axes between C and D for some genes. N=3 for each sample type. *P<0.05 based on an unpaired t-test using Welch's correction, error bars represent s.e.m.