Fig. 1.

Gap27 reduced Cx43 protein levels in normal 3T3 fibroblasts. (A,B) Representative images of cells with no treatment (NT), 100 µM scrambled peptide (SP) treated, or treated with increasing doses of Gap27 for (A) 2 h or (B) 6 h. Cells were immunolabeled for Cx43 (green, white arrows) and nuclei (blue) identified by Höechst. (C,D) Cx43 labelling intensity was quantified at (C) 2 h and (D) 6 h. (E) Western blots of Cx43 protein levels at 6 h after Gap27 treatment. Alpha tubulin was included as a loading control housekeeping protein. (F) Intensity of the Cx43 band was presented in ratio to alpha tubulin. Data are means±s.e.m. (n=3). *P<0.01, **P<0.001, ^#P<0.05 compared with NT, one-way ANOVA. Scale bar=50 µm.