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High concentration of Gap27 reduced GJIC in normal 3T3 fibroblasts. (A) Representative images of FRAP experiment in cells treated with Gap27 (30, 300 µM), scrambled peptide (300 µM), or no-treatment for 2 h. The bleached cell was circled in red. (B) The recovery profiles of fluorescence after FRAP. Data are means±s.e.m. (n=3). **P<0.01 compared with NT, one-way ANOVA. Scale bar=10 µm.
