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. 2015 Oct 15;4(11):1473–1480. doi: 10.1242/bio.013573

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — PI uptake in low confluence cells subjected to OGDR insult and Gap27 incubation. (A) Representative images of 3T3 fibroblasts after OGDR insult and Gap27 treatment, up to 5 h after OGDR. Live cells were identified by Calcein AM (green) and cells with open CxHC showed permeation of PI (red). (B) Quantification of PI-positive cells, as a percentage of Calcein-AM positive cells in the first frame of time-lapse. Data are means±s.e.m. (n=4). **P<0.01, ***P<0.001, ****P<0.0001 compare with No OGDR, one-way ANOVA. Scale bar=100 µm.