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. 2015 Oct 21;4(11):1518–1527. doi: 10.1242/bio.013961

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Lid is required to maintain GSC proliferation and prevent premature differentiation. (A) Percentage of PH3 positive GSCs in testes from males of the following genotypes: wt (7.4%); lid (2.7%); lid, UAS-DE-Cadherin; nos-Gal4 (1.7%); and lid, UAS-Stat92E; nos-Gal4 (6.8%). P-value calculated using Fisher's test. (B,C) Immunostaining using antibodies against Vasa (red), Arm (green), and α-spectrin (green) in wt (B) and lid (C) testes. Arrow points to round spectrosome in (B). 4-cell spermatogonia cyst at the hub is outlined (yellow dotted line) in (C). Hub area is outlined (white dotted line). (D-E′) Immunostaining with antibodies against Vasa (red), Arm (magenta), and Bam-GFP (green) in Bam-GFP (D,D′) and lid; Bam-GFP (E,E′) testes. Arrows point to Bam-expressing GSCs in (E′). Hub area is outlined (white dotted line). Scale bars: 10 µm.