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. 2015 Oct 21;4(11):1518–1527. doi: 10.1242/bio.013961

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Lid regulates Stat92E in GSCs to maintain their proliferation and prevent premature differentiation. (A-E) Immunostaining using antibodies against Arm (blue) and Vasa (green) in wt (A), lid (B), and lid; Stat92E/+ testes (C). Arrows point to spermatocytes. 8-cell spermatogonia cyst is outlined (yellow dotted line) in (C). (D) lid, UAS-Stat92E; nos-Gal4 with rescued GSC number. (E) nos-Gal4/UAS-lid shmiRNA; UAS-Stat92E testis with rescued GSC number. Dots indicate GSCs which we identified as Vasa-labeled cells in direct contact with the hub. Hub area is outlined (white dotted line). (F) Quantification of average GSC number in testes from males of the following genotypes: wt (9.7±1.6); lid (6.03±1.4); lid, UAS-Stat92E; nos-Gal4 (10.5±1.9); nos-Gal4 control (9.5±1.6); nos-Gal4/UAS-lid shmiRNA (5.9±1.1); nos-Gal4/UAS-lid shmiRNA; UAS-Stat92E (10±1.1) and nos-Gal4; UAS-Stat92E (7.3±0.9). P-values calculated using Student's t-test. Error bars represent s.d. (G,H) Immunostaining with antibodies against Vasa (red), FasIII (green), and α-spectrin (green) in nos-Gal4 (G) and nos-Gal4; UAS-Stat shmiRNA (H) testes. Arrows point to round spectrosome in (G) and branched fusome in (H). Scale bars: 10 µm.