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. 2015 Oct 15;4(11):1481–1489. doi: 10.1242/bio.014159

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Ecad-GFP is immobilized through adhesive and non-adhesive interactions at cell junctions. (A) FRAP analysis of PDAC cells expressing either Ecad-GFP (top row) or a mutant unable to form cis-, trans-, or actin interactions (ΔEC1ΔCyt-GFP, bottom row). Bar: 5 µm. (B) Average fluorescence recovery curves for Ecad-GFP, ΔEC1ΔCyt-GFP and GFP-F. (C) The half-time of recovery (T_1/2) and immobile fraction (F_i) were derived from exponential functions fitted to individual FRAP curves, error bars represent s.e.m. Note that although the graph shows F_i and T_1/2, T_1/2 is actually a property of F_m. See supplementary material Table S2 for a complete list of FRAP parameters.