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. 2015 Dec 31;7(1):1. doi: 10.3390/genes7010001

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immuno-dot blot using antibody specific for 5-methylcytosine. Lane A represents 100 ng of methylated DNA from the plasmid pUC (A1), 100 ng of unmethylated DNA from pUC (A2), 625 ng of human genomic DNA (A3), 250 ng of 5-hydroxymethylated DNA from Staphylococcus aureus (A4). Lane B—200 ng of genomic DNAs from D. pulex juveniles (B1, B2), preadults (B3, B4), adults I (B5, B6), and adults II (B7, B8); even numbers—individuals hatched form parthenogenetic eggs; odd numbers—individuals hatched from ephippial eggs.