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. 2016 Jan 27;6:19827. doi: 10.1038/srep19827

Figure 2. Overview of the difference in spindle size and in vitro maturational time course in pig and mouse oocytes.

Figure 2

(A) Mouse oocytes at the GV, GVBD, MI and MII stages were stained with PI and anti-α-tublin antibody to detect mouse meiotic spindle shape and size. (B) Pig oocytes at the GV, GVBD, MI and MII stages were stained with PI and anti-α-tubulin antibody to detect pig meiotic spindle shape and size. (C) Percentages of mouse oocyte GVBDs (0 h: 0%; 1 h: 39.53 ± 6.10%; 2 h: 74.49 ± 6.26%; 3 h: 78.57 ± 2.18%; 4 h: 79.41 ± 2.03%; 5 h: 79.41 ± 2.03%; 6 h: 79.41 ± 2.03%) were observed each hour from 0–6 h of in vitro culture, and percentages of pig oocyte GVBDs (6 h: 0%; 10 h: 3.53 ± 1.22; 15 h: 7.11 ± 1.24; 20 h: 54.44 ± 5.09; 24 h: 72.67 ± 3.48) were observed at 6 h, 10 h, 15 h, 20 hand 24 h of in vitro culture. (D) Percentages of mouse oocytes with first polar body extrusion (8 h: 0%; 10 h: 15.70 ± 0.93%; 11 h: 65.22 ± 7.25%; 12 h: 75.56 ± 5.18%; 13 h: 79.94 ± 4.38%; 14 h: 79.94 ± 4.38%; 15 h: 79.94 ± 4.38%; 16 h: 79.94 ± 4.38%) were observed one or two hours from 8 h to 16 h of in vitro culture, and percentages of pig oocytes with first polar body extrusion (20 h: 0%; 30 h: 11.01 ± 6.27%; 44 h: 45.55 ± 3.85%; 48 h: 50.14 ± 4.07%) were observed at 20 h, 30 h, 44 hand 48 h of in vitro culture (bar = 30 μm).