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. 2016 Jan 5;7:10269. doi: 10.1038/ncomms10269

Figure 2. Inhibition of HSP90 activity with the specific inhibitor GDA suppresses plant responses to auxin.

Figure 2

(a–d) Four- or five-day-old Col-0 wild-type seedlings were transferred to plates with IAA and/or GDA for various times (a, 3 days; b–d, 4 days). For d, we plotted primary root growth after transfer to IAA plates. The concentration of IAA used was: (a) 2 μM, (b,c) 1 μM and (d) 0.02 μM. The concentration of GDA used is: (a) 5 μM, (b,c) 1, 3 and 10 μM and (d) 2 μM. Error bars are s.d., n=10–12. *Significant difference with mock, **significant difference with IAA. (e) Five-day-old DR5:GUS seedlings were treated with IAA (2 μM) with and without GDA (10 μM) for 24 h and stained for GUS. Scale bars, 5 cm (c) and 200 μm (e).