Figure 1.

CRCs to isoprenaline and mirabegron in mouse isolated urethra smooth muscle. Responses to isoprenaline (A) were evaluated with or without either L‐748,337 (30 μM) alone or in combination with atenolol (10 μM) and ICI‐118,551 (10 μM). Responses to mirabegron were evaluated with or without atenolol (10 μM; B), ICI‐118,551 (10 μM; C) or L‐748,337 (1–30 μM; D). (D) Dotted lines indicate the urethral relaxations at the levels of 25% and 75%. (E) The Schild plot for L‐748,337 at mirabegron's pEC₂₅. (F) CRCs to mirabegron in urethra pre‐contracted with KCl (80 mM), arginine‐vasopressin (60 nM) and endothelin‐1 (100 nM). Relaxations were calculated relative to the maximal changes from the contraction produced by phenylephrine (10 μM) in each urethral ring, which was taken as 100%. Data are presented as mean ± SEM (n = 3–4 different animals for each curve).