Figure 3.

Concentration–response curves to phenylephrine (PE) in mouse isolated urethra with or without different concentrations of mirabegron. Urethral preparations were not pretreated (A) or pretreated (B) with a cocktail of inhibitors containing yohimbine (100 nM), propranolol (100 nM), L‐748,337 (10 μM), 17β‐estradiol (10 μM) and desipramine (100 nM). (C) Schild plots for mirabegron with and without the cocktail of inhibitors. (D) Representative original traces for PE‐induced urethral contractions in a preparation pretreated with the cocktail of inhibitors. Data are presented as mean ± SEM (n = 4–6).