Figure 2. Cre models with low beige adipocyte labelling.

(a–h) Two-month-old male Cre mice (Myf5-Cre and Myogenin (MyoG)-Cre); R26R^RFP mouse models were maintained at RT (23 °C) or cold exposed (6 °C) for 7 days. Subcutaneous inguinal adipose depot explants were imaged for direct RFP fluorescence or sectioned and immunostained for reporter (RFP, red) and UCP1 (green) (a–d). Two-month-old male, inducible Cre mice (PDGFRα-Cre^ERT2 and Myh11-Cre^ERT2); R26R^RFP mice were administered one dose of TM on 2 consecutive days. Mice were maintained at RT or cold exposed for 7 days. Subcutaneous inguinal adipose depot explants were imaged or sectioned and immunostained for reporter (RFP, red) and UCP1 (green) (e–h). (i,j) Myh11-Cre^ERT2; R26R^RFP were also housed in the cold or RT for 14 days. Subcutaneous inguinal adipose depot explants were imaged or sectioned and immunostained for reporter (RFP, red) and UCP1 (green). Quantification of UCP1+ beige adipocytes that were RFP+ is denoted next to the IHC image. Images are representative from n=4 mice per group replicated twice. Scale bar, 10 μm (a,c,e,g,i). Scale bar, 100 μm (b,d,f,h,j).