Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jan 5;7:10184. doi: 10.1038/ncomms10184

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) Merged (H2B-GFP and RFP) whole-mount images of denoted adipose depots and other tissues from 2-month-old AdipoTrak (PPARγ^τTA,TRE-Cre); TRE-H2B-GFP; R26R^RFP male mice and control (PPARγ^tTA; RFP) mice. BAT, classical brown adipose tissue; IGW, inguinal; MWAT, mesenteric white adipose tissue; PGW, perigonadal; PSCW, periscapular; RPW, retroperitoneal. Scale bar, 10 μm. (b) Image of control (R26R^RFP) and Myf5-Cre; RFP male mice. Please note the RFP fluorescence was easily observed in regular room lighting from the Myf5-Cre; RFP mouse. (c) Fluorescence microscopic whole-mount images of RFP fluorescence from denoted adipose depots from 2-month-old Myf5-Cre; RFP RT mice. Scale bar, 10 μm. (d) Two-month-old Myf5-Cre; RFP male mice were maintained at RT or cold exposed for 7 days. Histological sections from periscapular (top: PSCW) and retroperitoneal (bottom: RPW) adipose depots were examined for RFP fluorescence and UCP1 (green) expression. Cell nuclei were counterstained with DAPI (blue). Quantification of UCP1+ beige adipocytes that were RFP+ is denoted next to the IHC image. (e) Adipose depot cells from 2-month-old Myf5-Cre; RFP mice were analysed by flow cytometry for co-expression of RFP with the indicated genes. (f) Adipose depot cells from 2-month-old Myf5-Cre; RFP male mice were separated into RFP+ and RFP− fractions using FACS and examined for mRNA expression of denoted genes using qPCR. ND, not detected. Data are means±s.e.m. (n=4–5 mice per group replicated twice). Scale bar, 100 μm. Student's t-test, *P<0.05, RFP+ compared with RFP−.