Figure 7. Endogenous SMA and SMA-driven reporter expression profile in beige adipocytes.

(a) Subcutaneous adipose depots from 2-month-old, cold-exposed wild-type mice were immunostained for endogenous SMA and UCP1 expression, and nuclei detected with DAPI. (b) Subcutaneous adipose depots from 2-month-old, cold-exposed wild-type male mice were IHC stained for endogenous SMA and UCP1. (c,d) Two-month-old uninduced (no TM) UCP1-Cre^ERT2 (c) or Adiponectin-Cre^ERT2 (d) male mice were cold exposed. After 7 days of cold exposure, mice were administered TM and maintained at RT for 24 h. Subcutaneous inguinal adipose depots were immunostained for RFP and UCP1 (green); nuclei were highlighted with DAPI. (e) Subcutaneous adipose depots from cold-exposed uninduced SMA-Cre^ERT2; RFP were immunostained for RFP and UCP1 (green); nuclei were highlighted with DAPI. (f) Uninduced SMA-Cre^ERT2; RFP mice were cold exposed for 7 days; mice were then pulsed with TM and housed at RT for 24 h. Sections from the subcutaneous inguinal adipose depots were immunostained for RFP and UCP1 (green). Cell nuclei were counterstained with DAPI (blue). (g) Uninduced SMA-rtTA; RFP mice were exposed to cold for 7 days. Subcutaneous inguinal adipose depot were sectioned and immunostained for RFP and UCP1 (green). Cell nuclei were counterstained with DAPI (blue). (h) Uninduced SMA-rtTA; RFP mice were cold exposed for 7 days and then administered Dox for 24 h and maintained at RT. Subcutaneous inguinal adipose depot sections were analysed for RFP (red), UCP1 (green) and DAPI (blue).