. 2016 Jan 27;6:19748. doi: 10.1038/srep19748

Table 1. Candidate reference genes, primer sequences, and amplicon characteristics of C. sinensis.

Gene symbol	Gene name	Arabidopsis homolog gene	Primer sequence (5′–3′) forward/reverse	Amplicon length (bp)	E (%)	Tm °C
CsACT7	Actin7 gene	AT5G09810	TTGGATTCTGGGGATGGTGTTAGC	195	102.6	84.5
CsACT7	Actin7 gene	AT5G09810	AGCAAGTTTCTCTTTCACATCACGG	195	102.6	84.5
CsEF-1α	Elongation factor-1α gene	AT5G60390	TTGACAAGCGTGTGATTGAGAGGT	177	107.0	84
CsEF-1α	Elongation factor-1α gene	AT5G60390	GGGCATCAATGACAGTGCAGTAGTA	177	107.0	84
CseIF-4α	Eukaryotic translation initiation factor 4α-1 gene	AT3G13920	TGAGTTACTTGTGGCTGATGGAGAA	145	107.5	82
CseIF-4α	Eukaryotic translation initiation factor 4α-1 gene	AT3G13920	CCTTTGCTGAATTGCAGACGGCTT	145	107.5	82
CsGAPDH	Glyceraldehyde-3-phosphate dehydrogenase gene	AT1G42970	CCCTCTAAGTCCTCTCACTCCTCCT	158	95.5	84
CsGAPDH	Glyceraldehyde-3-phosphate dehydrogenase gene	AT1G42970	GTTGCTTGGGCAGCCACTACAT	158	95.5	84
CsPP2A	Protein phosphatase 2A gene	AT3G21650	AAGAAGAGGAACTGGCGACGGAAC	153	102.9	83.5
CsPP2A	Protein phosphatase 2A gene	AT3G21650	CAAACAGGTCCAGCAAACGCAAC	153	102.9	83.5
CsSAND	SAND family protein gene	AT2G28390	GCCTGAACCGTCTTCTGTGGAGT	184	101.9	87
CsSAND	SAND family protein gene	AT2G28390	CTCAATCTCAGACACACTGGTGCTA	184	101.9	87
CsTBP	TATA-box binding protein gene	AT1G55520	GGCGGATCAAGTGTTGGAAGGGAG	166	107.0	85
CsTBP	TATA-box binding protein gene	AT1G55520	ACGCTTGGGATTGTATTCGGCATTA	166	107.0	85
CsTIP41	Tap42-interacting protein of 41 kDa gene	AT4G34270	TGGAGTTGGAAGTGGACGAGACCGA	176	103.6	87
CsTIP41	Tap42-interacting protein of 41 kDa gene	AT4G34270	CTCTGGAAAGTGGGATGTTTGAAGC	176	103.6	87
CsTUB	Tubulin beta-6 gene	AT5G12250	AATGAGGCTTCTTGTGGGAGGTTTG	147	107.0	86.5
CsTUB	Tubulin beta-6 gene	AT5G12250	GTTATTTCCAGCACCAGACTGACCG	147	107.0	86.5