Table 2. Gene expression stability ranked by geNorm, NormFinder, and BestKeeper.
Group | Rank | geNorm | NormFinder | BestKeeper | ||||
---|---|---|---|---|---|---|---|---|
Gene | Stability | Gene | Stability | Gene | SD [±Cq] | CV [%Cq] | ||
Developmental stages | 1 | CseIF-4α | 0.19 | CsTBP | 0.01 | CsACT7 | 0.42 | 1.84 |
2 | CsEF-1α | 0.19 | CsTIP41 | 0.04 | CsTIP41 | 0.45 | 1.81 | |
3 | CsTBP | 0.22 | CseIF-4α | 0.05 | CsGAPDH | 0.57 | 2.63 | |
4 | CsSAND | 0.28 | CsPP2A | 0.06 | CseIF-4α | 0.58 | 2.36 | |
5 | CsPP2A | 0.31 | CsSAND | 0.09 | CsEF-1α | 0.61 | 3.00 | |
6 | CsTIP41 | 0.36 | CsEF-1α | 0.12 | CsTBP | 0.66 | 2.58 | |
7 | CsACT7 | 0.46 | CsACT7 | 0.16 | CsSAND | 0.75 | 2.91 | |
8 | CsGAPDH | 0.63 | CsTUB | 0.17 | CsPP2A | 0.82 | 3.06 | |
9 | CsTUB | 0.90 | CsGAPDH | 0.27 | CsTUB | 1.89 | 7.94 | |
Hormone stimuli | 1 | CsPP2A | 0.19 | CsEF-1α | 0.07 | CseIF-4α | 0.32 | 1.29 |
2 | CsTBP | 0.19 | CsTBP | 0.07 | CsEF-1α | 0.34 | 1.69 | |
3 | CsSAND | 0.23 | CseIF-4α | 0.07 | CsGAPDH | 0.39 | 1.84 | |
4 | CseIF-4α | 0.28 | CsPP2A | 0.08 | CsTIP41 | 0.39 | 1.54 | |
5 | CsEF-1α | 0.29 | CsSAND | 0.09 | CsTBP | 0.41 | 1.62 | |
6 | CsTUB | 0.33 | CsTIP41 | 0.09 | CsACT7 | 0.45 | 1.94 | |
7 | CsTIP41 | 0.37 | CsACT7 | 0.09 | CsPP2A | 0.47 | 1.77 | |
8 | CsACT7 | 0.41 | CsTUB | 0.13 | CsTUB | 0.51 | 2.22 | |
9 | CsGAPDH | 0.47 | CsGAPDH | 0.23 | CsSAND | 0.52 | 1.99 | |
Total | 1 | CsEF-1α | 0.20 | CsTBP | 0.06 | CseIF-4α | 0.45 | 1.82 |
2 | CseIF-4α | 0.20 | CseIF-4α | 0.06 | CsTIP41 | 0.45 | 1.77 | |
3 | CsTBP | 0.26 | CsTIP41 | 0.07 | CsEF-1α | 0.48 | 2.34 | |
4 | CsPP2A | 0.31 | CsPP2A | 0.08 | CsACT7 | 0.50 | 2.16 | |
5 | CsSAND | 0.33 | CsSAND | 0.10 | CsTBP | 0.51 | 2.01 | |
6 | CsTIP41 | 0.37 | CsEF-1α | 0.10 | CsGAPDH | 0.52 | 2.41 | |
7 | CsACT7 | 0.46 | CsACT7 | 0.14 | CsPP2A | 0.63 | 2.34 | |
8 | CsGAPDH | 0.59 | CsTUB | 0.15 | CsSAND | 0.67 | 2.57 | |
9 | CsTUB | 0.78 | CsGAPDH | 0.26 | CsTUB | 1.14 | 4.89 |
SD [±Cq]: standard deviation of the Cq; CV [%Cq]: coefficient of variance expressed as a percentage of the Cq level.