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. 2016 Jan 27;6:19738. doi: 10.1038/srep19738

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(a) Map of pGsdf2Agfp. The 2.5 kb β-actin promoter, gsdf, gfp and self-cleavage sequence 2A as well as flanking restriction sites are indicated. (b) Genotype and phenotype of F0 adults. (c–f) Transgenic GFP expression in F1 embryos at 1 dpf (c) or 4 dpf (d) and adult heart (e) and testis (f). (g,h) Cross sections of adult gonads immunostained with an anti-GFP antibody, showing testicular structure and presence of many sperm of a wildtype XY gonad (g) and a XX gonad transgenic for pGsdf2Agfp. (g’,h’) Larger magnification of areas framed in (g) and (h), highlighting the absence in (g’) and presence in (h’) of GFP (arrows). sm, sperm. Scale bar, 50 μm.