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. 2016 Jan 27;6:19738. doi: 10.1038/srep19738

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Copyright © 2016, Macmillan Publishers Limited

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Hatching F3 embryos (9 dpf) from crosses between gsdf^+/− fish and Vg fish bearing GFP-labeled germ cells were analyzed by confocal microscopy and then genotyped. GFP-positive PGCs (green) are clearly visible in the gonad. (a–c), XX gonads, showing similar abundance of PGCs in normal (a), gsdf^+/− (b) and gsdf^−/− (c) embryos. (d–f), XY gonads, highlighting fewer PGCs in normal (d) and gsdf^+/− embryos (e) than XX embryos (a–c), whereas the PGC number in gsdf^−/− embryos (f) is comparable to that in XX embryos (a–c). Numbers of PGCs are presented as means ± SD with number of embryos examined are given in parenthesis. Scale bar, 50 μm.