Figure 6. Asynchronous expansion of higher- and lower-avidity clonotypes.

(a) Flow cytometric detection of CD45.2⁺ donor EVα3 Rag1^−/− Emv2^−/− CD4⁺ T cells and CD45.1⁺CD45.2⁺ host CD4⁺ T cells following transfer either on the day of infection (d0 of infection, d0–7) or 10 days after infection (d10 of infection, d10–17) with FV or FV/LDV. In both setups, T-cell expansion was analysed 7 days after transfer (n=5–9). (b) Absolute number of env-reactive donor EVα3 CD4⁺ T cells recovered from the spleens of the same recipients described in a over the course of FV infection or FV/LDV coinfection (nd, not detected). (c) Absolute number of Vα2 or Vα3 env-reactive donor EF4.1 CD4⁺ T cells recovered from the spleens of FV/LDV infected recipients 7 days after transfer either on the day of infection (d0–7) or 10 days after infection (d10–17). (d) Numbers of EVα3 T cells recovered 7 days after transfer from the spleens of lymphocyte-deficient Rag1^−/− Emv2^−/− recipients that were either left uninfected or were F-MLV-B-infected. Each symbol represents an individual mouse.